Analysis Note

Negative ControlHepG2 cells or normal skin

Positive ControlSK-BR-3 cells or breast carcinoma tissue

Application

Frozen Sections (2.5 g/ml)Immunoblotting (2-3 g/ml, see application references)Immunofluorescence (2.5 g/ml)Immunoprecipitation (1 g/sample, see application references)Paraffin Sections (2.5 g/ml, pressure cooker, heat or trypsin pre-treatment required, see application references)

General description

Recognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells.

Anti-c-ErbB2/c-Neu (Ab-3), mouse monoclonal, clone 3B5, recognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells. It is validated for WB, IF, IP, and IHC on frozen and paraffin sections.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0.Ag14 cells (see application references). Recognizes the ~185 kDa c-Erb2/c-Neu protein.

Immunogen

a synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For paraffin sections, we recommend pre-treating with a pressure cooker, but trypsin and heat will also work well. Including 0.05% saponin during staining of paraffin sections may help reduce background. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribriform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.

Mamcs, J.R., et al. 1994. Ann. Surg.219, 332.Tetu, B., et al. 1994. Cancer73, 2359.DiFiore, P.P., et al. 1987. Science237, 178.Slamon, D.J., et al. 1987. Science235, 177.Varley, J.M., et al. 1987. Oncogene1, 423.Bargmann, C.I., et al. 1986. Nature319, 226.Yamamoto, T., et al. 1986. Nature319, 230.Blick, M., et al. 1984. Blood64, 1234.Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Warning

Toxicity: Standard Handling (A)